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   Table of Contents     
Year : 2014  |  Volume : 7  |  Issue : 4  |  Page : 189-197
Principles and methods of preparation of platelet-rich plasma: A review and author's perspective

Department of Dermatology, Lokmanya Tilak Municipal (LTM) Medical College and Hospital, Mumbai, Maharashtra, India

Click here for correspondence address and email

Date of Web Publication4-Feb-2015


The utility of platelet-rich plasma (PRP) has spanned various fields of dermatology from chronic ulcer management to trichology and aesthetics, due to its role in wound healing. Though PRP is being used over a long time, there is still confusion over proper terminology to define, classify and describe the different variations of platelet concentrates. There is also a wide variation in the reported protocols for standardization and preparation of PRP, in addition to lack of accurate characterization of the tested products in most articles on the topic. Additionally, the high cost of commercially available PRP kits, precludes its use over a larger population.
In this article, we review the principles and preparation methods of PRP based on available literature and place our perspective in standardizing a safe, simple protocol that can be followed to obtain an optimal consistent platelet yield.

Keywords: Platelet-rich plasma, preparation, principles, standardization

How to cite this article:
Dhurat R, Sukesh M S. Principles and methods of preparation of platelet-rich plasma: A review and author's perspective. J Cutan Aesthet Surg 2014;7:189-97

How to cite this URL:
Dhurat R, Sukesh M S. Principles and methods of preparation of platelet-rich plasma: A review and author's perspective. J Cutan Aesthet Surg [serial online] 2014 [cited 2022 Oct 2];7:189-97. Available from:

   Background Top

Platelets are cytoplasmic fragments of megakaryocytes, formed in the marrow [1],[2] and approximately 2 μm in diameter. They contain more than 30 bioactive proteins, many of which have a fundamental role in hemostasis or tissue healing. [3]

Seven fundamental protein growth factors that are actively secreted by platelet initiate all wound healing process [Table 1]. [4] PRP also includes three proteins in blood known to act as cell adhesion molecules: Fibrin, fibronectin and vitronectin. [5]
Table 1: Platelet growth factors and their specific characteristics[4]

Click here to view

Activation causes the granules present in platelets to fuse to its cell membrane (also called degranulation) where the secretory proteins (e.g. PDGF, TGF-β etc.) [6] are transformed to a bioactive state by the addition of histones and carbohydrate side chains. [3] The active proteins are then secreted, binding to transmembrane receptors of target cells, which include mesenchymal stem cells, osteoblasts, fibroblasts, endothelial cells and epidermal cells. These agonists bound transmembrane receptors then activate an intracellular signal protein that causes the expression of a gene sequence that directs cellular proliferation, matrix formation, osteoid production, collagen synthesis etc.thus provoking tissue repair and tissue regeneration. [3],[4],[5],[6],[7],[8]

The active secretion of these growth factors by platelets begins within 10 min after activation, with more than 95% of the pre-synthesized growth factors secreted within 1 h. [9]

Marx proposed that platelet count of 10 lakh/ml in 5 mL of PRP, as a working definition of PRP, based on the scientific proof of bone and soft tissue healing enhancement. [9] There is a scarcity of studies stating the concentration at which optimal stimulation occurs. Rughetti et al. [10] studied the relationship between the concentration of platelets in platelet gel and changes in the functional activity of human endothelial cells. The proliferation of endothelial cells and its migration and the invasion of endothelial cells occurred in a bell-shaped manner. The authors found that the stimulation for proliferation of endothelial cells peak at 1.25 × 10 6 and angiogenesis at 1.5 × 10 6 platelets/mL, respectively. This signifies the fact that a PRP platelet count 1 million/mL has become the working definition for therapeutic PRP and also reasons out the criticism on not getting the expected best results of PRP, which might be due to lower concentrations of platelets.

   Classification Top

The literature on PRP is considerable, but the published results are often contradictory. It is very difficult to sort and interpret the available data, due to a large number of preparation techniques, terminologies, forms of these materials, and the endless list of potential applications.

According to the classification proposed by Ehrenfest et al. (2009), four main families of preparations can be defined, depending on their cell content and fibrin architecture. [11]

  1. Pure Platelet-Rich Plasma (P-PRP) or leucocyte-poor PRP products are preparations without leucocytes and with a low-density fibrin network after activation.
  2. Leucocyte- and PRP (L-PRP) products are preparations with leucocytes and with a low-density fibrin network after activation. It is in this family that the largest number of commercial or experimental systems exist. Particularly, many automated protocols have been developed in the last years, requiring the use of specific kits that allow minimum handling of the blood samples and maximum standardization of the preparations.
  3. Pure platelet-rich fibrin (P-PRF) or leucocyte-poor platelet-rich fibrin preparations are without leucocytes and with a high-density fibrin network. These products only exist in a strongly activated gel form, and cannot be injected or used like traditional fibrin glues.
  4. Leucocyte- and platelet-rich fibrin (L-PRF) or second-generation PRP products are preparations with leucocytes and with a high-density fibrin network.

This classification system was largely cited, advocated, and validated by a multi-disciplinary consensus conference published in 2012. [12]

   Preparation of PRP Top

PRP is obtained from a sample of patients' blood drawn at the time of treatment. A 30 cc venous blood draw will yield 3-5 cc of PRP depending on the baseline platelet count of an individual, the device used, and the technique employed. The blood draw occurs with the addition of an anticoagulant, such as citrate dextrose A to prevent platelet activation prior to its use. The authors employ a specialized 'table top cold centrifuge' device. Preparation-related costs are significantly lower than with commercial kits.

   Principles of PRP Preparation Top

PRP is prepared by a process known as differential centrifugation. In differential centrifugation, acceleration force is adjusted to sediment certain cellular constituents based on different specific gravity.

There are many ways of preparing PRP. It can be prepared by the PRP method or by the buffy-coat method.

In the PRP method, an initial centrifugation to separate red blood cells (RBC) is followed by a second centrifugation to concentrate platelets, which are suspended in the smallest final plasma volume. In [Figure 1], flow chart describes a double centrifugation process of PRP. WB (whole blood) is initially collected in tubes that contain anticoagulants. The first spin step is performed at constant acceleration to separate RBCs from the remaining WB volume. After the first spin step, the WB separates into three layers: an upper layer that contains mostly platelets and WBC, an intermediate thin layer that is known as the buffy coat and that is rich in WBCs, and a bottom layer that consists mostly of RBCs. For the production of pure PRP (P-PRP), upper layer and superficial buffy coat are transferred to an empty sterile tube. For the production of leucocyte rich PRP (L-PRP), the entire layer of buffy coat and few RBCs are transferred. The second spin step is then performed. 'g' for second spin should be just adequate to aid in formation of soft pellets (erythrocyte-platelet) at the bottom of the tube. The upper portion of the volume that is composed mostly of PPP (platelet-poor plasma) is removed. Pellets are homogenized in lower 1/3rd (5 ml of plasma) to create the PRP (Platelet-Rich Plasma).
Figure 1: Flowchart describing preparation of PRP

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In the buffy coat method, whole blood (WB) is centrifuged at a 'high speed' with subsequent collection of the buffy coat. A buffy coat contains high concentration of leucocytes.

From small volume of WB (10 mL), a very thin layer of a buffy coat can be produced. The difficulty lies in separating this thin buffy coat layer that contains mainly white blood cells (WBCs) and platelets, from the underlying RBC layer.

Procedure [13],[14]

PRP method

  1. Obtain WB by venipuncture in acid citrate dextrose (ACD) tubes
  2. Do not chill the blood at any time before or during platelet separation.
  3. Centrifuge the blood using a 'soft' spin.
  4. Transfer the supernatant plasma containing platelets into another sterile tube (without anticoagulant).
  5. Centrifuge tube at a higher speed (a hard spin ) to obtain a platelet concentrate.
  6. The lower 1/3 rd is PRP and upper 2/3 rd is platelet-poor plasma (PPP). At the bottom of the tube, platelet pellets are formed.
  7. Remove PPP and suspend the platelet pellets in a minimum quantity of plasma (2-4 mL) by gently shaking the tube.

Buffy coat method

  1. WB should be stored at 20°C to 24°C before centrifugation.
  2. Centrifuge WB at a 'high' speed.
  3. Three layers are formed because of its density: The bottom layer consisting of RBCs, the middle layer consisting of platelets and WBCs and the top PPP layer.
  4. Remove supernatant plasma from the top of the container.
  5. Transfer the buffy-coat layer to another sterile tube.
  6. Centrifuge at low speed to separate WBCs or use leucocyte filtration filter.

There is no consensus on whether or not platelets must be previously activated before their application and with which agonist. Some authors activate platelets with thrombin or calcium, whereas others apply platelets without being previously activated, arguing that better results are obtained. [15]

   Commercially Available PRP Kits Top

There are many PRP systems commercially marketed, which facilitate the preparation of ready to apply platelet-rich suspensions in a reproducible manner. All operate on a small volume of drawn blood (20-60 mL) and on the principle of centrifugation. These systems differ widely in their ability to collect and concentrate platelets depending on the method and time of its centrifugation. As a result, suspensions of different concentration of platelets and leucocytes are obtained. Differences in the concentrations in platelets and WBCs influence the diversity of growth factors concentration. It is difficult to assess which kit for PRP preparation is better and which is worse.

PRP devices can be usually divided into lower (2.5-3 times baseline concentration) and higher (5-9 times baseline concentration) systems. The high-yielding devices include Biomet GPS II and III (platelet count 3-8×); Harvest SmartPRep 2 APC+ (4-6×); ArterioCyte-Medtronic Magellan (3-7×). The lower concentration systems include Arthrex ACP (2-3×), Cascade PPR therapy (1-1.5×), and PRGF by Boitech Institute Vitoria, Spain (2-3×).Regen PRP (Regen Laboratory, Mollens, Switzerland). [6],[16],[17],[18],[19]

   Comparison of Studies of Preparation Top

PRP is prepared by centrifugation varying the relative centrifugal force, temperature and time. It has been seen that the two-step procedure renders the highest output.

Preparation procedures are also relevant, as shown by studies of the chondro-inductive and osteo-inductive potential of PRP. [20]

There are numerous protocols in the current literature that describe the optimal conditions for centrifugation [Table 2]. However, these various protocols have been optimized with respect to different variables of the process, such as volume and sampling of processed WB, number of spins, time period of centrifugation, and range of centrifugal acceleration. Considering the complexity of an autologous product such as PRP and the need for quality control in clinical applications, it is crucial to demonstrate procedure's ability to reproduce consistent results.
Table 2: Comparison of various protocols for platelet yield

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Despite these variations, all protocols follow a generic sequence that consists of blood collection, an initial centrifugation to separate RBCs, subsequent centrifugations to concentrate platelets, and other components and an activation of the sample by adding a platelet agonist.

Amable et al. [21] studied variations in relative centrifugal force (RCF), temperature, and time for optimizing conditions for platelet isolation and quantification of cytokines and growth factors in PRP before and after platelet activation.

Peripheral blood from 22 healthy male and female volunteer donors (20-54 years old) was collected using 4.5 mL blood collection tubes containing 0.5 mL citrate solution (VacutainerW, Ref: 369714; BD Biosciences). Blood samples were run by choosing RCF from 240 to 400×g, time from 8 to 19 min and temperature from 8° to 16°C. All steps were performed in a refrigerated centrifuge (certified Jouan Br4i, Saint-Herblain, Loire-Atlantique, France).

The best performance was obtained using parameters of 300×g for 5 min at 12°C and 240×g for 8 min at 16°C for 1 st spin. The second spin of 700 × g for 17 min was chosen since it allowed a lower platelet loss into the PPP fraction and produced a pellet that was easily resuspended. [21]

Amanda et al. [22] demonstrated that the processing of 3.5 mL of blood at 100×g for 10 min (1 st spin), 400×g for 10 min (2 nd spin) and withdrawing 2/3 of remnant plasma, promoted high platelet recovery (70%-80%) and concentration (5×) maintaining platelet integrity and viability. Authors believe that time and acceleration are the fundamental parameters that define the composition of the PRP sample after the first spin step. Longer time periods slightly increased platelet recovery and decreased the concentrations of WBC in the upper layer. Therefore, time could be a control parameter when low levels of WBC, such as granulocytes and lymphocytes, are required in the PRP sample.

Various factors contribute to platelet concentration gradient such as the size of platelets, the biological difference among individuals and hematocrit variability.

However, this gradient is more critical after the second spin step because some erythrocytes are inevitably present in the volume that was transferred from the first spin. The presence of these remaining RBCs can generate a pellet at the bottom of the tube, which adsorb platelets and WBCs on its surface. The manual mixing for a short period of time is insufficient to completely resuspend the platelets, and a large variability in platelet counting is observed. Approximately 20% of platelets remained adsorbed in the RBC pellet. [22]

Kahn et al. [23] determined that a centrifugal acceleration of 3731×g for a period of 4 min was the optimal condition for obtaining the highest platelet concentration from 478 mL of WB.

The highest platelet recovery efficiency obtained by Slichter and Harker [24] was 80%, using a sample of 250-450 mL of WB centrifuged at 1000×g for a period of 9 min. It was observed that a subsequent centrifugation step of 3000×g for a period of 20 min decreased the platelet viability.

Landesberg et al. obtained PRP samples that had approximately 3.2 times the concentration of the WB baseline. The centrifugation procedure processed 5 mL of WB for two spins at 200×g for 10 min per spin. [25]

Jo et al. examined the effect of the centrifugation time and gravitational force (g) on the platelet recovery ratio of PRP. Two-step centrifugations for preparing PRP were used in 39 subjects. WB was centrifuged from 500×g to 1900×g at 200×g increments for 5 min and from 100×g to 1300×g at 200×g increments for 10 min. In the step 2, platelets in the separated plasma were concentrated at 1000×g for 15 min, 1500×g for 15 min, 2000×g for 5 min and 3000×g for 5 min. They achieved better efficiency (92%) by applying an acceleration of 900×g for 5 min for the first spin step. A total of 9 mL of WB were processed, and the platelet concentration was measured to be 310.7 ± 78.5 × 10 3 /mm 3 . The maximum efficiency for the second spin step (84%) was obtained by applying 1500×g for 15 min. The platelet concentration was 633.2 ± 91.6 × 10 3 /mm 3 , which was 4.2 times greater than the baseline concentration. [26]

Bausset et al. found that a centrifugation of 130×g or 250×g for a period of 15 min was optimal when performing a procedure that involved two spins. A platelet concentration factor of 3.47 was obtained from the 8.5 mL WB processed, and 2.0 mL of plasma was processed in the second spin step. [27]

Tamimi et al. [28] compared two methods for obtaining PRP: Double centrifugation (ACE system; Surgical Supply and Surgical Science Systems, Brockton, MA, USA) and single centrifugation (Nahita System; Nahita, Navarra, Spain). Three test tubes of 8.5 mL WB each were introduced into an ACE centrifuge machine and subjected to a force of 160×g (1300 rpm) for 10 min. For second centrifugation, 400×g force (2000 rpm) for 10 min was applied. For Nahita system blood was extracted into 3.5-mL citrated tubes (Venojet; Terumo MR, Tokyo, Japan) containing 0.5 mL of trisodium citrate, citrate, and ACD as anticoagulants. Test tubes were centrifuged with a 280×g force (1500 rpm) for 7 min. Platelet concentration from the ACE and Nahita systems were (336%) and (227%), respectively.

Mazzocca et al. [29] analyzed three protocols for preparing PRP samples with different compositions: A low platelet (382 × 10 3 /mm 3 ) and low WBC (0.6 × 10 3 /mm 3 ) process with one spin step at 1500 rpm for 5 min (10 mL WB); a high platelet (940 × 103/mm 3 ) and high WBC (17 × 103/mm 3 ) process with one spin step at 3200 rpm for 15 min (27 mL WB); and a double-spin process (1500 rpm for 5 min and 6300 rpm for 20 min) that produced a higher platelet concentration (472 × 103/mm 3 ) and lower WBC (1.5 × 103/mm 3 ).

Anitua et al. [30] used only one centrifugation spin step and collected the volume immediately above the erythrocyte layer. Blood was collected on sterile tubes (4.5 mL) containing 3.8% (w/v) trisodium citrate, then centrifuged at 460×g for 8 min (PRGF System1, B.T.I. Biotechnology Institute, Vitoria-Gasteiz, Spain). This protocol obtained a platelet concentration factor of 2.67 above the baseline value.

Dugrillon et al. [31] reported that the number of platelets is not always proportional to the growth factors' quantity, so more attention should be given to the quality of PRP than to the number of platelets concentrated. Their study proved that the TGF-β1 and platelet concentration are proportionally related to the centrifugation forces when the forces are less than 800×g. TGF-β1 becomes inversely related to the centrifugal force when forces are above 800×g.

Araki et al. [32] concluded that optimized protocol for PRP preparation was centrifugation of WB at 230-270×g for 10 min, which also contained a low number of WBCs (4.1%-5.8% of WB). WBCs appeared to be precipitated when the centrifugation force was ≥840×g.

In their protocol, WB (40-72 mL) was drawn by venipuncture, collected, and divided into 7.5-mL aliquots in 15-mL conical tubes and centrifuged conical tubes (15 mL; BD Falcon) . Tubes were centrifuged at 20°C in a refrigerated centrifuge (Kubota 5900; Kubota Co.). For the second spin step, an acceleration of 2300×g for 10 min was applied. The platelet concentration factor was 7.4 times greater than the baseline after removing approximately 1/10 of the PPP and adding ethylenediaminetetraacetic acid (EDTA) as an anticoagulant.

Many other studies [33],[34] specified centrifugal accelerations in rotations per minute (rpm) instead of in ×g, complicating the task of comparing and reproducing their results.

In a recent study by Kececi et al., [35] the first spin (soft spin) protocol was chosen as 250-270×g for 10 min. At the second spin, centrifugation force was varied from 300×g, 500×g, 750×g, 1000×g, 1500×g and 2000×g for 10 min. The platelet concentration factor increased as the centrifugal force of the second spin increased as 1.92-fold, 2.16-fold, 2.80-fold, 3.48-fold, 3.67-fold, and 3.76-fold after a 10-min second centrifugation at 300×g, 500×g, 750×g, 1000×g, 1500×g and 2000×g, respectively. The authors opined that obtaining a definite platelet concentration might be possible by adjusting centrifugation force individually according to the personal baseline value.

   Authors' Perspective Top

There are various protocols described in the literature for preparation of PRP. Centrifugal acceleration, number of spins, time and distance between the particles and the rotor to the volume of processed WB are critical for optimization of PRP. Each laboratory must standardize its protocol. A handmade standard PRP could be prepared reliably and cost effectively without using a commercial kit. The platelet concentration factor can be changed with the centrifugation force applied in PRP preparation.

Factors influencing PRP yield

Various factors influence the yield of PRP such as draw of blood; speed, time and temperature of centrifugation and use of anticoagulants.

Draw of blood

The clotting process is influenced from the time of the draw. To avoid unintentional activation of platelets, most protocols use large bore needles (>22) to draw the blood. [17]

In a study by Waters and Roberts. [18] using two cell-salvage devices and two table top devices over the course of 260 clinical cases, they found that there was a downward trend in platelet counts with longer draw time.


The earth's gravitational force is sufficient to separate many types of particles over time. A tube of anti-coagulated WB left standing on a bench top will eventually separate into plasma, RBC and WBC fractions. However, the length of time required precludes this manner of separation for most applications. In addition, the potential degradation of biological compounds during prolonged storage means faster separation techniques are needed. Hence, to accelerate sedimentation, the effect of gravity is amplified using 'centrifugal force' provided by a centrifuge and can be many thousand times the force of gravity.

Separation of cellular constituents within blood can be achieved by a process known as differential centrifugation. In differential centrifugation, acceleration force is adjusted to sediment certain cellular constituents and leave others in suspension. In centrifugation, RCF is the force required to separate two phases, this force also called relative centrifugal field.

It is expressed as multiples of the earth's gravitational field (g). By accelerating the g, speedy sedimentation can be achieved.

'g' is the actual force exerted on the contents of the spinning rotor, which separates the aqueous solutions in the centrifuge. Revolutions per minute (rpm) is calculated using the following equation. [36]


g = (1.118 × 10 -5 ) R S2

Where 'g' is the RCF, R is the radius of the rotor (from centre of rotor to sample) in centimetres and S is the speed of the centrifuge in revolutions per minute.

It is important to remember that calculation of RCF is dependent on the radius of the centrifuge rotor used. The same centrifuge machine with different rotors can produce different acceleration forces.


Temperature during processing is crucial to prevent platelet activation. AABB manual recommends 21°C-24°C for centrifugation of blood for obtaining PRP. [13]

Macey et al. [37] also stated that cooling may retard platelet activation and this may be essential in obtaining PRP with viable platelets.

Many authors have used a temperature level of 12°C-16°C during centrifugation for best platelet recovery.

This is germane to those who use an ordinary centrifuge to develop PRP, which are mainly developed for diagnostic purposes and not for PRP processing and hence may not produce a sufficient platelet yield.


The importance lies in choosing an anticoagulant capable of preserving the platelets' best possible functionality, integrity, and morphology.

With regard to the type of anticoagulant for use, most authors agree on not using EDTA because it could damage the platelet membrane. Therefore, anticoagulants with citrate and dextrose of sodium citrate are recommended. [38]

The authors compared the effects of sodium citrate and ACD-A on platelet aggregation, pH and extracellular iCa concentration. The anticoagulant ACD-A is the choice for collection of platelets by apheresis, whereas trisodium citrate (3.2% or 3.8%) is the anticoagulant most commonly used for diagnostic evaluations of platelets. Trisodium citrate and ACD-A solutions differ markedly in pH, with ACD-A having a pH of 4.9 and 3.8% sodium citrate having a pH of 7.8. In addition, the citrate ion concentration in ACD-A is 15.6 mg/mL, whereas 3.8% sodium citrate contains 24.4 mg of citrate ion/mL. It has been reported. In several species that alterations in the pH and extracellular iCa concentration of PRP can affect platelet aggregation in vitro, with aggregation typically impaired at acidic pH and lower extracellular iCa concentrations. [39],[40]

Alternatively citrate phosphate dextrose-adenine) can be used. It is similar to ACD-A but has fewer supportive ingredients and, therefore, is 10% less effective in maintaining platelet viability. [9]

Activation of PRP

PRP activation prior to injection is another parameter that requires further discussion. PRP can be activated exogenously by thrombin, calcium chloride or mechanical trauma. Collagen is a natural activator of PRP, thus when PRP is used in soft tissue, it does not need to be exogenously activated. [17]

Once PRP is activated, (fibrinogen-fibrin) a fibrin network begins to form, solidifying the plasma and creating a fibrin clot or membrane.

According to Weibrich et al., [41] there are no significant changes in the platelet concentration or on the concentration of growth factors in relation to age and gender, although there are studies that report that the hematocrit and total platelet count influence the platelet concentration of the PRP. [42]

   Helpful Tips for Standardization of PRP Procedure Top

The clinician needs to determine how much PRP he would need to generate for the particular procedure.

As previously stated, many factors influence PRP yield. The following points are helpful in obtaining an optimum PRP concentrate.

  1. Draw blood in anticoagulant vacutainer tubes using BD Eclipse™ blood collection needle (Ref: 368607; BD Biosciences, India) [Figure 2]. Set apart 1-2 mL for baseline cell counting including RBCs, platelets, WBCs and hematocrit. Donor percent hematocrit (the ratio of the volume of RBCs to the total volume of blood) is relevant to plasma yield optimization. Blood with a low percent hematocrit has more plasma available thereby diluting the concentration of platelets in plasma.
    Figure 2: Venipuncture using BD Eclipse™ blood collection needle

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  2. Blood samples that are collected in ACD tubes should be inverted 5-10 times for proper mixing of the anticoagulant and blood. If the tube is not mixed, small fibrin clots may form, causing a falsely decreased platelet count.
  3. After 1 st spin, measure the platelet count in RBCs and the supernatant to ensure optimal separation of platelets from the WB.
  4. If this does not happen, change the parameters like rpm and time.
  5. After first spin, if large volume of plasma is obtained, then it would need higher 'g' to concentrate platelets at the bottom of the tube (law of velocity).
  6. After 2 nd spin, measure the platelet count in PPP and PRP after adequately racking the tubes.
  7. If higher concentration of platelets in platelet poor plasma (upper layer) or lower concentration in PRP (bottom layer) is observed, then the parameters are not optimal.
  8. PRP must be separated from the PPP soon after centrifugation because the concentrated platelets will slowly diffuse into the PPP over time and would reduce the platelet count of the PRP preparation. [3]
  9. For counting the platelets in the final PRP concentrate, it must be re-suspended for at least 5-10 min to allow for equal distribution of platelets before counting. [31]

To obtain a yield of platelet in PRP more than 10 lakhs/mL, all variables like rpm, time and temperature should be standardized and also consistency of platelet concentrate has to be maintained over the period After several attempts with various permutations & combinations, the authors found that at 900g x 5 mins for 1st centrifugation and 1000g x 10 mins for 2nd centrifugation at 16° C in a refrigerated centrifuge (RemiCM8plus; Remiworld, India), the PRP yield was found to be optimum. Authors have consistently recorded platelet count exceeding 10 lakhs/ml using these parameters [Figure 3].
Figure 3: Consistency of PRP yield

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   Conclusion Top

0The utility of PRP is increasing in the field of dermatology and aesthetics. Though commercially marketed PRP systems offer ready-to-apply platelet-rich suspensions in a sterile manner, high cost, limited volume of drawn blood, differences in centrifugation guidelines, wide variation in platelet concentration, makes it difficult for the clinician to choose amongst the readily available PRP kits.

There are many protocols for preparation of PRP each having its own standardized parameters and claimed results, as mentioned in the review. It is advisable to standardize individual preparation protocols, which are cost-effective and easy to adapt in clinical settings.

Understanding of basic principles of centrifugation is of paramount importance in preparation of PRP. Repeated methodology trials by 'trial and error approach', strict vigilance at maintaining sterility and regular cross-checking of the platelet values are a must to obtain consistent results.

   References Top

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DOI: 10.4103/0974-2077.150734

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73 Use of Platelet-Rich Plasma in Treating Low Back Pain: A Review of the Current Literature
Mirza Zain Baig, Umm E Hani Abdullah, Aun Muhammad, Abeer Aziz, Maryam Jamil Syed, Aneela Darbar
Asian Spine Journal. 2021; 15(1): 117
[Pubmed] | [DOI]
74 Treatment of knee osteoarthritic pain with platelet-rich plasma: a systematic review of clinical studies
Rohit Aiyer, Selaiman Noori, Frank Schirripa, Michael Schirripa, Talal Aboud, Sameer Jain, Amitabh Gulati, Vinay Puttanniah, Semih Gungor, Corey Hunter
Pain Management. 2021; 11(4): 419
[Pubmed] | [DOI]
75 Platelet-rich plasma in interventional dermatology and trichology: How far have we come?
Suruchi Garg, Anuva Bansal
Cosmoderma. 2021; 1: 12
[Pubmed] | [DOI]
76 Rapid Wound Healing and Acne Scar Improvement After Ablative Fractional Carbon Dioxide Laser Treatment Combined with the Application of Platelet-Lyophilized Treatment (PLT)
Chieh-Chen Huang, Haw-Yueh Thong
Clinical, Cosmetic and Investigational Dermatology. 2021; Volume 14: 715
[Pubmed] | [DOI]
Arquivos de Gastroenterologia. 2021; 58(2): 185
[Pubmed] | [DOI]
Pablo Morón-Elorza, Carlos Rojo-Solís, Mario Soriano-Navarro, Teresa Álvaro-Álvarez, Mónica Valls-Torres, Daniel García-Párraga
Journal of Zoo and Wildlife Medicine. 2021; 52(3)
[Pubmed] | [DOI]
79 PRP therapy in chronic diffuse alopecia areata – A case report
Kashish Tyagi, Sheilly Kapoor, Ishani Mohapatra, Komal Sharma
IP Indian Journal of Clinical and Experimental Dermatology. 2021; 7(4): 363
[Pubmed] | [DOI]
80 Efficacy of platelet-rich plasma injection in comparison to physical therapy for treatment of chronic partial supraspinatus tear
Sarah Saad Mohamed Ahmed Serya, Nanis Onsy Neseem, Reham M. Shaat, Khaled Nour.A., Mohammad K. Senna
Egyptian Rheumatology and Rehabilitation. 2021; 48(1)
[Pubmed] | [DOI]
81 Using platelet-rich plasma for skin rejuvenation
Claudia McGloin
Journal of Aesthetic Nursing. 2021; 10(10): 452
[Pubmed] | [DOI]
82 Platelet-rich plasma: a narrative review
Thomas Collins, Dinesh Alexander, Bilal Barkatali
EFORT Open Reviews. 2021; 6(4): 225
[Pubmed] | [DOI]
83 The Biological Use of Platelet-Rich Plasma in Skeletal Muscle Injury and Repair
Jordan Boivin, Rachael Tolsma, Peter Awad, Keith Kenter, Yong Li
The American Journal of Sports Medicine. 2021; : 0363546521
[Pubmed] | [DOI]
84 In-vitro production and pre-validation of lyophilized canine platelet-rich plasma for therapeutic use
Natália P.P. Freitas, Maria Márcia M.S. Maior, Beatriz A.P. Silva, Marcus R.L. Bezerra, José F. Nunes, Saul G. Neto, Kaio C.S. Tavares, Leonardo T. Martins
Pesquisa Veterinária Brasileira. 2021; 41
[Pubmed] | [DOI]
85 Effect of Combined Leukocyte-Poor Platelet-Rich Plasma and Hyaluronic Acid on Bone Marrow–Derived Mesenchymal Stem Cell and Chondrocyte Metabolism
Alexander M. Satin, Jolanta B. Norelli, Nicholas A. Sgaglione, Daniel A. Grande
CARTILAGE. 2021; 13(2_suppl): 267S
[Pubmed] | [DOI]
86 Intra-articular platelet-rich plasma injections versus intra-articular corticosteroid injections for symptomatic management of knee osteoarthritis: systematic review and meta-analysis
Michael McLarnon, Neil Heron
BMC Musculoskeletal Disorders. 2021; 22(1)
[Pubmed] | [DOI]
87 Red blood cells and their releasates compromise bone marrow-derived human mesenchymal stem/stromal cell survival in vitro
Ryan Christopher Dregalla, Jessica Ann Herrera, Edward Jeffery Donner
Stem Cell Research & Therapy. 2021; 12(1)
[Pubmed] | [DOI]
88 Comparing the efficacy of dual Platelet-Rich Plasma (PRP) and Hyaluronic Acid (HA) therapy with PRP-alone therapy in the treatment of knee osteoarthritis: a systematic review and meta-analysis
Angeline Ai Ling Aw, Jun Jie Leeu, Xinyu Tao, Hamid Rahmatullah Bin Abd Razak
Journal of Experimental Orthopaedics. 2021; 8(1)
[Pubmed] | [DOI]
89 Stress Urinary Incontinence: Treatment With Platelet-Rich-Plasma Injection and Placement of Polydioxanone Threads—A Pilot Study
Ariel Luksenburg, Juan J. Barcia, Roberto Sergio, Santiago Fernandez, Marco A. Pelosi, Marco A. Pelosi
The American Journal of Cosmetic Surgery. 2021; : 0748806821
[Pubmed] | [DOI]
90 Allogeneic Platelet-Rich Plasma: Is It Safe and Effective for Wound Repair?
Shiva Akbarzadeh, Maxwell B. McKenzie, Md. Mostafizur Rahman, Heather Cleland
European Surgical Research. 2021; 62(1): 1
[Pubmed] | [DOI]
91 Effect of upstream priming on transient downstream platelet-substrate interactions
Elizabeth Anne Pumford, Shekh Mojibur Rahman, Vladimir Hlady
Colloids and Surfaces B: Biointerfaces. 2021; 206: 111925
[Pubmed] | [DOI]
92 In-thrombus thrombin secretion: a new diagnostic marker of atrial fibrillation in cryptogenic stroke
Ze'ev Itsekson Hayosh, Eiman Abu Bandora, Natalia Shelestovich, Maya Nulman, Mati Bakon, Gal Yaniv, Boris Khaitovitch, Shmuel Balan, Alexandra Gerasimova, Tali Drori, Stefan Mausbach, Yvonne Schwammenthal, Arnon Afek, Joab Chapman, Efrat Shavit Stein, David Orion
Journal of NeuroInterventional Surgery. 2021; 13(9): 799
[Pubmed] | [DOI]
93 Effects of platelet-rich plasma on the healing of sternal wounds: A meta-analysis
Dan Yao, Guang Feng, Fan Zhao, Daifeng Hao
Wound Repair and Regeneration. 2021; 29(1): 153
[Pubmed] | [DOI]
94 Variation in platelet-rich plasma compositions used for wound healing indications
Ivo Bijl, Dirk Korte, Esther Middelkoop
Wound Repair and Regeneration. 2021; 29(2): 284
[Pubmed] | [DOI]
95 Possible clinical efficacy and tolerability of platelet-rich plasma with atopic dermatitis
Ayman Yosef, Nada Elkady, Fathia Khattab
Journal of Cosmetic Dermatology. 2021; 20(10): 3264
[Pubmed] | [DOI]
96 Platelet-rich plasma is a promising therapy for melasma
Ankita Tuknayat, Mala Bhalla, Gurvinder Pal Thami
Journal of Cosmetic Dermatology. 2021; 20(8): 2431
[Pubmed] | [DOI]
97 Platelet-rich plasma in hair loss—Mechanism, preparation, and classification
Sajin Alexander, Ilse Horo, Sobha Johnson, Sheela Daniel
Journal of Cosmetic Dermatology. 2021;
[Pubmed] | [DOI]
98 Platelet-Rich Plasma: Evolving Role in Plastic Surgery
Edward S. Chamata, Erica L. Bartlett, David Weir, Rod J. Rohrich
Plastic & Reconstructive Surgery. 2021; 147(1): 219
[Pubmed] | [DOI]
99 Autologous Concentrated Growth Factors Combined with Topical Minoxidil for the Treatment of Male Androgenetic Alopecia: A Randomized Controlled Clinical Trial
Poh-Ching Tan, Pei-Qi Zhang, Yun Xie, Yi-Ming Gao, Qing-Feng Li, Shuang-Bai Zhou, Qing Liu, Kai Liu
Facial Plastic Surgery & Aesthetic Medicine. 2021; 23(4): 255
[Pubmed] | [DOI]
100 The Emperor Has No Platelets: Minimal Effects in an Alopecia Split-Scalp Study Unsurprising as Platelet-Rich Plasma Was Actually Platelet-Poor
Patrick K Yam
Aesthetic Surgery Journal. 2021;
[Pubmed] | [DOI]
101 Emerging follicular activation strategies to treat women with poor ovarian response and primary ovarian insufficiency
Andres Reig, Sonia Herraiz, Antonio Pellicer, Emre Seli
Current Opinion in Obstetrics & Gynecology. 2021; 33(3): 241
[Pubmed] | [DOI]
102 Resumed ovarian function and pregnancy in early menopausal women by whole dimension subcortical ovarian administration of platelet-rich plasma and gonadotropins
Chao Chin Hsu, Isabel Hsu, Leonard Hsu, Yi Jen Chiu, Sonam Dorjee
Menopause. 2021; 28(6): 660
[Pubmed] | [DOI]
103 Arachidonic Acid-Dependent Pathway Inhibition in Platelets: its Role in Multiple Injury-Induced Coagulopathy and the Potential Mechanisms
Yao Tang, Sunhua Huang, Wenhao Lin, Ke Wen, Zhexuan Lin, Ming Han
Shock. 2021; 55(1): 121
[Pubmed] | [DOI]
104 Platelet-rich plasma—Where do we stand today? A critical narrative review and analysis
Gulhima Arora, Sandeep Arora
Dermatologic Therapy. 2021; 34(1)
[Pubmed] | [DOI]
105 Effect of platelet-rich plasma on the outcome of mini-punch grafting procedure in localized stable vitiligo: Clinical evaluation and relation to lesional basic fibroblast growth factor
Samar Abdallah M. Salem, Tabarek Abdulkhaleq Fezeaa, Nashwa El Khazragy, Marwa Y. Soltan
Dermatologic Therapy. 2021; 34(2)
[Pubmed] | [DOI]
106 Evaluation of the efficacy and safety of platelet rich plasma injection in treatment of rosacea
Maysa T. Ghoz, Dareen A. Mohamed, Zainab A. Ibrahim, Ghada Fawzy Rezk Hassan
Dermatologic Therapy. 2021; 34(5)
[Pubmed] | [DOI]
107 Subcision, chemical peels, and platelet-rich plasma: Combination approaches for the treatment of striae distensae
Howyda M. Ebrahim, Amira Salem, Tagreed Salah, Fatma Eldesoky, Hala Mohamed Morsi
Dermatologic Therapy. 2021;
[Pubmed] | [DOI]
108 Desenvolvimento de um dispositivo para obter plasma rico em plaquetas (PRP)
Marília de Lima Mota, Ronald Bispo Barreto, Bárbara Ramos Leite, Beatriz Costa Cardoso Dourado Cavalcante
Revista Brasileira de Ortopedia. 2021;
[Pubmed] | [DOI]
109 The Effect of Calcium Gluconate on Platelet Rich Plasma Activation for VEGF-A Expression of Human Dental Pulp Stem Cells
Anggraini Margono, Dini Asrianti Bagio, Indah Julianto, Endang Suprastiwi
European Journal of Dentistry. 2021;
[Pubmed] | [DOI]
110 Methylation-Sensitive Loop-Mediated Isothermal Amplification (LAMP): Nucleic Acid Methylation Detection through LAMP with Mobile Fluorescence Readout
Jacob Amos Hambalek, Janay Elise Kong, Calvin Brown, Hector Enrique Munoz, Thomas Horn, Michael Bogumil, Eleni Quick, Aydogan Ozcan, Dino Di Carlo
ACS Sensors. 2021; 6(9): 3242
[Pubmed] | [DOI]
111 Blood apheresis technologies – a critical review on challenges towards efficient blood separation and treatment
Christine Jurene O. Bacal, James W. Maina, Harshal H. Nandurkar, Maryam Khaleel, Rosanne Guijt, Yung Chang, Karen M. Dwyer, Ludovic F. Dumée
Materials Advances. 2021; 2(22): 7210
[Pubmed] | [DOI]
112 AIRMESS – Academy of International Regenerative Medicine & Surgery Societies: recommendations in the use of platelet-rich plasma (PRP), autologous stem cell-based therapy (ASC-BT) in androgenetic alopecia and wound healing
Pietro Gentile, Rubina Alves, John P. Cole, Katarina Andjelkov, Thierry Van Helmelryck, Jonathan Fernandez, Angelo Trivisonno, Lemierre Guillaume, Alexis Verpaele, Patrick Tonnard, Jeremy Magalon, Guy Magalon, Sophie Menkes
Expert Opinion on Biological Therapy. 2021; 21(11): 1443
[Pubmed] | [DOI]
113 Application of platelet-rich plasma in traumatic bone infections
Ruohui Tang, Shaochuan Wang, Jing Yang, Tong Wu, Jun Fei
Expert Review of Anti-infective Therapy. 2021; 19(7): 867
[Pubmed] | [DOI]
114 Evidence-based indications of platelet-rich plasma therapy
Shyla Gupta, Anna Paliczak, Diego Delgado
Expert Review of Hematology. 2021; 14(1): 97
[Pubmed] | [DOI]
115 The use of platelet-rich products for skin graft donor site healing: a systematic review and meta-analysis
Christopher F. Brewer, Alexander Smith, Ben H. Miranda
Journal of Plastic Surgery and Hand Surgery. 2021; 55(3): 133
[Pubmed] | [DOI]
116 Platelet-Derived Products in Veterinary Medicine: A New Trend or an Effective Therapy?
Carla S. Soares, Pedro S. Babo, Rui L. Reis, Pedro P. Carvalho, Manuela E. Gomes
Trends in Biotechnology. 2021; 39(3): 225
[Pubmed] | [DOI]
117 Efficacy of topical application of autologous platelet-rich plasma in adult tonsillectomy patients: a randomised control study
S Nanditha, S Gopalakrishnan, P Karthikeyan, S Singh Bakshi
The Journal of Laryngology & Otology. 2021; 135(6): 539
[Pubmed] | [DOI]
118 Biophysical Phenomenon-Based Separation of Platelet-Poor Plasma from Blood
Vijai Laxmi, Suhas S. Joshi, Amit Agrawal
Industrial & Engineering Chemistry Research. 2021; 60(19): 7464
[Pubmed] | [DOI]
119 Evidence for the use of PRP in chronic midsubstance Achilles tendinopathy: A systematic review with meta-analysis
An-Katrien Nauwelaers, Loïc Van Oost, Koen Peers
Foot and Ankle Surgery. 2021; 27(5): 486
[Pubmed] | [DOI]
120 Biocompatibility study of nanocomposite titanium boron nitride (TiBN) thin films for orthopedic implant applications
Anusha Thampi V.V., V.I. Chukwuike, Dmitry V. Shtansky, B. Subramanian
Surface and Coatings Technology. 2021; 410: 126968
[Pubmed] | [DOI]
121 Promoting limbal stem cells proliferation and maintenance using post-thaw human amniotic membranes fortified by platelet lysate
Samira Karami, Sahar Balagholi, Mozhgan Rezaei Kanavi, Shaban Alizadeh, Rasul Dabbaghi, Mahtab Sayadi
Gene Reports. 2021; 23: 101084
[Pubmed] | [DOI]
122 Faster calculation of scalp area in androgenetic alopecia before platelet-rich plasma therapy using the mobile application ImageMeter
Sandip Agrawal, Rachita Dhurat, Aseem Sharma, Kiran Chahal, Smita Ghate, Mithali Jage
Journal of the American Academy of Dermatology. 2021; 85(4): e249
[Pubmed] | [DOI]
123 A response to “Inconsistent platelet-rich plasma product from the Food and Drug Administration cleared devices: A retrospective review of clinic data”
Shilpi Khetarpal
Journal of the American Academy of Dermatology. 2021; 85(3): e177
[Pubmed] | [DOI]
124 Comparative analysis of leucocyte poor vs leucocyte rich platelet-rich plasma in the management of lateral epicondylitis: Systematic review & meta-analysis of randomised controlled trials
Sathish Muthu, Sandeep Patel, Preethi Selvaraj, Madhan Jeyaraman
Journal of Clinical Orthopaedics and Trauma. 2021; 19: 96
[Pubmed] | [DOI]
125 Platelet enhancement by Carica papaya L. leaf fractions in cyclophosphamide induced thrombocytopenic rats is due to elevated expression of CD110 receptor on megakaryocytes
C. Nandini, SubbaRao V. Madhunapantula, Venugopal R. Bovilla, Mohammad Ali, K. Mruthunjaya, Manjula N. Santhepete, K. Jayashree
Journal of Ethnopharmacology. 2021; 275: 114074
[Pubmed] | [DOI]
126 Autologous platelet gel improves outcomes in tubularized incised plate repair of hypospadias
Karam Elsayem, Ahmed S Darwish, Amr Abdelhamid AbouZeid, Noha Kamel, Mohamed M Dahab, Osama El-Naggar
Journal of Pediatric Surgery. 2021;
[Pubmed] | [DOI]
127 Response Regarding: The Effects of Platelet-Rich Plasma to Decrease the Risk of Seroma Formation After Mastectomy and Axillary Dissection
Akin Calisir, Ilhan Ece, Serdar Yormaz, Bayram Colak, Halil Kirazli, Mustafa Sahin
Journal of Surgical Research. 2021; 259: 575
[Pubmed] | [DOI]
128 Platelet-Rich Plasma (PRP) Improves Erectile Function: A Double-Blind, Randomized, Placebo-Controlled Clinical Trial
Evangelos Poulios, Ioannis Mykoniatis, Nikolaos Pyrgidis, Filimon Zilotis, Paraskevi Kapoteli, Dimitrios Kotsiris, Dimitrios Kalyvianakis, Dimitrios Hatzichristou
The Journal of Sexual Medicine. 2021; 18(5): 926
[Pubmed] | [DOI]
129 Short-Term Voice Improvement after Repeated Office-Based Platelet-Rich Plasma PRP Injection in Patients with Vocal Fold Scar, Sulcus, and Atrophy
Peak Woo, Thomas Murry
Journal of Voice. 2021;
[Pubmed] | [DOI]
130 Laryngeal Applications of Platelet Rich Plasma and Platelet Poor Plasma: A Systematic Review
Aishwarya Suresh, Bailey Balouch, Vishnu V. Martha, Robert T. Sataloff
Journal of Voice. 2021;
[Pubmed] | [DOI]
131 Demineralized freeze-dried bone allograft vs biphasic calcium phosphate: A comparison of two graft materials in sinus augmentation procedures - A pilot study
Nishtha Gadkari, Shilpa Bawane, Ratima Chopra, Kalyani Bhate, Pushkar Waknis, Pradnya Kakodkar, Deepak Kulkarni, Paresh Kale
Advances in Oral and Maxillofacial Surgery. 2021; 4: 100177
[Pubmed] | [DOI]
132 New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density
Subhaini Jakfar, Tzu-Chieh Lin, Shinn-Chih Wu, Yao-Horng Wang, Yu-Jun Sun, Minal Thacker, Li-Xin Liu, Feng-Huei Lin
Bioactive Materials. 2021; 6(10): 3528
[Pubmed] | [DOI]
133 Prophylactic use of platelet-rich plasma for post-spinal low back pain following gynecological surgery: a randomized clinical trial
Ghada Mohammad Abo Elfadl, Abdelraheem Mahmoud Elawamy, Abualauon Mohamed Abedalmohsen, Azza Abo Elfadl El Sayed, Mustafa Bahloul, Eman Ahmed Ismail
Brazilian Journal of Anesthesiology (English Edition). 2021;
[Pubmed] | [DOI]
134 Biodegradable cellulose-based superabsorbent as potent hemostatic agent
Ahmad Mahmoodzadeh, Jafarsadegh Moghaddas, Seyedhosein Jarolmasjed, Abbas Ebrahimi Kalan, Mahdi Edalati, Roya Salehi
Chemical Engineering Journal. 2021; 418: 129252
[Pubmed] | [DOI]
135 A narrative review of platelet-rich plasma (PRP) in reproductive medicine
Fady I. Sharara, Latashia-Lika Lelea, Sara Rahman, Jordan S. Klebanoff, Gaby N. Moawad
Journal of Assisted Reproduction and Genetics. 2021; 38(5): 1003
[Pubmed] | [DOI]
136 Effectiveness of Platelet-Rich Plasma in the Prevention of Chlamydia-Induced Hydrosalpinx in a Murine Model
Sheena M. Rippentrop, Zhi Huo, Zengzi Zhou, Francisco Zaldana, Stephanie Hernandez, Randal D. Robinson, Guangming Zhong
Reproductive Sciences. 2021; 28(4): 1031
[Pubmed] | [DOI]
137 Role of Triple Injection Platelet-Rich Plasma for Osteoarthritis Knees: A 2 Years Follow-Up Study
Najmul Huda, Mir Shahid ul Islam, Sandeep Bishnoi, Hemant Kumar, Shubham Aggarwal, Aijaz Ahmad Ganai
Indian Journal of Orthopaedics. 2021;
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138 Platelet-inspired therapeutics: current status, limitations, clinical implications, and future potential
Sarah M. Kola, Yahya E. Choonara, Pradeep Kumar, Pierre P. D. Kondiah, Viness Pillay
Drug Delivery and Translational Research. 2021; 11(1): 24
[Pubmed] | [DOI]
139 Rejuvenating the periorbital area using platelet-rich plasma: a systematic review and meta-analysis
Adam G. Evans, Mirjana G. Ivanic, Mina A. Botros, Rand W. Pope, Briana R. Halle, Gabriella E. Glassman, Rafaella Genova, Salam Al Kassis
Archives of Dermatological Research. 2021; 313(9): 711
[Pubmed] | [DOI]
140 Impact of platelet-rich plasma versus selenium in ameliorating induced toxicity in rat testis: histological, immunohistochemical, and molecular study
Walaa Mohamed Sayed, Ahmed Elzainy
Cell and Tissue Research. 2021; 385(1): 223
[Pubmed] | [DOI]
141 A retrospective analysis of a commercially available platelet-rich plasma kit during clinical use
Meredith H. Prysak, Christopher P. Kyriakides, Tyler A. Zukofsky, Stefan E. Reutter, Jennifer Cheng, Gregory E. Lutz
PM&R. 2021;
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142 Autologous matrix-induced chondrogenesis plus peripheral blood concentrate (AMIC+PBC) in chondral defects of the first metatarsophalangeal joint
Martinus Richter
Operative Orthopädie und Traumatologie. 2021; 33(6): 471
[Pubmed] | [DOI]
143 Platelet Rich Plasma Hybridized Adipose Transplant (PHAT) for the Treatment of Hair Loss: A Case Series
Benjamin Talei, Orr Shauly, Daniel Gould
Aesthetic Plastic Surgery. 2021;
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144 The future of skin tightening: Mechanical or biological?
Diane I. Duncan
Dermatological Reviews. 2020; 1(4): 128
[Pubmed] | [DOI]
145 Effects of submucosal PRP injection on wound healing in endonasal surgeries: an experimental study
Ugur Yildirim, Ozgur Kemal, Abdurrahman Aksoy, Efe Karaca, Ozlem Terzi, Sinan Atmaca
European Archives of Oto-Rhino-Laryngology. 2020; 277(6): 1681
[Pubmed] | [DOI]
146 Platelet Rich Plasma as a Treatment Method for Rotator Cuff Tears
Jack Hitchen, Nicholas M. Wragg, Maryam Shariatzadeh, Samantha L. Wilson
SN Comprehensive Clinical Medicine. 2020; 2(11): 2293
[Pubmed] | [DOI]
147 Riboflavin-mediated photooxidation to improve the characteristics of decellularized human arterial small diameter vascular grafts
Karl H. Schneider, Sabrina Rohringer, Barbara Kapeller, Christian Grasl, Herbert Kiss, Stefan Heber, Ingrid Walter, Andreas H. Teuschl, Bruno K. Podesser, Helga Bergmeister
Acta Biomaterialia. 2020; 116: 246
[Pubmed] | [DOI]
148 A humoral solution: Autologous blood products and tissue repair
Yuval Yogev
Cellular Immunology. 2020; 356: 104178
[Pubmed] | [DOI]
149 Matrix-associated stem cell transplantation (MAST) versus autologous matrix induced chondrogenesis plus peripheral blood concentrate (AMIC+PBC) in chondral defects of the first metatarsophalangeal joint – A clinical cohort analysis
Martinus Richter, Stefan Zech, Stefan Andreas Meissner, Issam Naef
Fuß & Sprunggelenk. 2020; 18(3): 217
[Pubmed] | [DOI]
150 Curcumin preconditioned human adipose derived stem cells co-transplanted with platelet rich plasma improve wound healing in diabetic rats
Hafiz Ghufran, Azra Mehmood, Maryam Azam, Hira Butt, Amna Ramzan, Muhammad Amin Yousaf, Asim Ejaz, Moazzam N. Tarar, Sheikh Riazuddin
Life Sciences. 2020; 257: 118091
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151 Electrical stimulation of whole blood for growth factor release and potential clinical implications
Allen L. Garner, Andrew S. Torres, Steve Klopman, Bogdan Neculaes
Medical Hypotheses. 2020; 143: 110105
[Pubmed] | [DOI]
152 Platelet-Rich Plasma Therapy for Male Sexual Dysfunction: Myth or Reality?
Maya V. Epifanova, Badri R. Gvasalia, Maksim A. Durashov, Sergey A. Artemenko
Sexual Medicine Reviews. 2020; 8(1): 106
[Pubmed] | [DOI]
153 Separation and Enrichment of Platelets from Whole Blood Using a PDMS-Based Passive Microdevice
Vijai Laxmi, Siddhartha Tripathi, Suhas S. Joshi, Amit Agrawal
Industrial & Engineering Chemistry Research. 2020; 59(10): 4792
[Pubmed] | [DOI]
154 Design Evolution and Performance Study of a Reliable Platelet-Rich Plasma Microdevice
Vijai Laxmi, Suhas S Joshi, Amit Agrawal
Industrial & Engineering Chemistry Research. 2020; 59(46): 20515
[Pubmed] | [DOI]
155 Enhancement of bone marrow aspirate concentrate with local self-healing corticotomies
Lei Shi, Boon Ching Tee, Hany Emam, Rachael Prokes, Peter Larsen, Zongyang Sun
Tissue and Cell. 2020; 66: 101383
[Pubmed] | [DOI]
156 Umbilical cord blood: Current uses for transfusion and regenerative medicine
Nicoletta Orlando, Claudio Pellegrino, Caterina Giovanna Valentini, Maria Bianchi, Ombretta Barbagallo, Sabrina Sparnacci, Franca Forni, Tecla Maria Fontana, Luciana Teofili
Transfusion and Apheresis Science. 2020; 59(5): 102952
[Pubmed] | [DOI]
157 Role of Platelet-Rich Plasma and the Science Behind Its Mechanism of Action
Nina Semsarzadeh, Shilpi Khetarpal
Advances in Cosmetic Surgery. 2020; 3(1): 1
[Pubmed] | [DOI]
158 Platelet-Rich Plasma Enhances Distal Flap Viability and Underlying Vascularity in a Radiated Rotational Flap Rodent Model
Brian Swendseid, Swar Vimawala, Tim Ortlip, Tony Richa, Nicholas Schmidt, Avery Walter, Ryan Heffelfinger, Adam Luginbuhl
Facial Plastic Surgery & Aesthetic Medicine. 2020; 22(3): 181
[Pubmed] | [DOI]
159 Platelet-rich plasma as a therapy for androgenic alopecia: a systematic review and meta-analysis
Adam G. Evans, James M. Mwangi, Rand W. Pope, Mirjana G. Ivanic, Mina A. Botros, Gabriella E. Glassman, F. Bennett Pearce, Salam Kassis
Journal of Dermatological Treatment. 2020; : 1
[Pubmed] | [DOI]
160 Platelet rich plasma injection versus topical erythromycin 2% in treatment of acne vulgaris
Zainab A. Ibrahim, Shereen F. Gheida, Amira R. El-Halaby, Ghada F. R. Hassan, Dareen A. Mohammed
Journal of Dermatological Treatment. 2020; : 1
[Pubmed] | [DOI]
161 Autologous activated platelet-rich plasma (AA-PRP) and non-activated (A-PRP) in hair growth: a retrospective, blinded, randomized evaluation in androgenetic alopecia
Pietro Gentile, Simone Garcovich
Expert Opinion on Biological Therapy. 2020; 20(3): 327
[Pubmed] | [DOI]
162 A randomized blinded retrospective study: the combined use of micro-needling technique, low-level laser therapy and autologous non-activated platelet-rich plasma improves hair re-growth in patients with androgenic alopecia
Pietro Gentile, Laura Dionisi, Jacopo Pizzicannella, Barbara de Angelis, Domenico de Fazio, Simone Garcovich
Expert Opinion on Biological Therapy. 2020; 20(9): 1099
[Pubmed] | [DOI]
163 The Combined Use of Curcumin and Platelet-Rich Plasma Enhances Axonal Regeneration in Acute Nerve Injuries: An Experimental Study in a Rat Model
Abraham Zavala, Peggy C. Martinez, Geovanna G. Gutierrez, Marino D. Vara, Wieslawa De Pawlikowski
Journal of Hand and Microsurgery. 2020;
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164 Tortuosity-powered microfluidic device for assessment of thrombosis and antithrombotic therapy in whole blood
David J. Luna, Navaneeth K. R. Pandian, Tanmay Mathur, Justin Bui, Pranav Gadangi, Vadim V. Kostousov, Shiu-Ki Rocky Hui, Jun Teruya, Abhishek Jain
Scientific Reports. 2020; 10(1)
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165 Fully automated platelet isolation on a centrifugal microfluidic device for molecular diagnostics
Chi-Ju Kim, Dong Yeob Ki, Juhee Park, Vijaya Sunkara, Tae-Hyeong Kim, YooHong Min, Yoon-Kyoung Cho
Lab on a Chip. 2020; 20(5): 949
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166 Platelet-rich plasma and regenerative dentistry
J Xu, L Gou, P Zhang, H Li, S Qiu
Australian Dental Journal. 2020; 65(2): 131
[Pubmed] | [DOI]
167 The Physician's Guide to Platelet-Rich Plasma in Dermatologic Surgery Part I: Definitions, Mechanisms of Action, and Technical Specifications
Amelia K. Hausauer, Shannon Humphrey
Dermatologic Surgery. 2020; 46(3): 348
[Pubmed] | [DOI]
168 The Effects of Adipose Derived Stromal Vascular Fraction and Platelet-Rich Plasma on Bone Healing of a Rat Model With Chronic Kidney Disease
Atilla Adnan Eyuboglu, Enver Arpaci, Abbas Albayati, Ahmet Cagri Uysal, Aysen Terzi, Sema Bozalioglu, Hale Turnaoglu, Cenk Balcik, Burak Ozkan, Nilgun Markal Ertas
Annals of Plastic Surgery. 2020; 85(3): 316
[Pubmed] | [DOI]
169 Heterogeneity in amount of growth factors secreted by platelets in platelet-rich plasma samples from alopecia patients
Javed A. Shaik, Nima Estharabadi, Ronda S. Farah, Maria K. Hordinsky
Experimental Dermatology. 2020; 29(10): 1004
[Pubmed] | [DOI]
170 Safety and efficacy of platelet-rich plasma vs carboxytherapy in the treatment of atrophic scars: A comparative clinical and histopathological study
Samia O. Nassar, Rania A. R. Eltatawy, Ghada F. R. Hassan
Dermatologic Therapy. 2020; 33(6)
[Pubmed] | [DOI]
171 Platelet-rich plasma for androgenetic alopecia: Efficacy differences between men and women
Aditya K. Gupta, Helen J. Renaud, Mary Bamimore
Dermatologic Therapy. 2020; 33(6)
[Pubmed] | [DOI]
172 T4 Pili Promote Colonization and Immune Evasion Phenotypes of Nonencapsulated M4 Streptococcus pyogenes
Yi-Hsuan Chen, Shao-Hui Li, Yao-Cheng Yang, Shu-Hao Hsu, Victor Nizet, Yung-Chi Chang, Indranil Biswas
mBio. 2020; 11(4)
[Pubmed] | [DOI]
173 Pulsed Radiofrequency Neuromodulation Contributes to Activation of Platelet-Rich Plasma inIn VitroConditions
Anna Michno, Zbigniew Kirkor, Ewelina Gojtowska, Marek Suchorzewski, Irmina Smietanska, Bartosz Bascik
Neuromodulation: Technology at the Neural Interface. 2020;
[Pubmed] | [DOI]
174 ???????????? ???????? ? ??????????? ??????
A. S. Sadullayev, M. V. Medvediev
????????? ??????? ?????????, ?????????? ?? ???????????. 2020; (1): 85
[Pubmed] | [DOI]
175 Quantification of Growth Factors and Fibronectin in Diverse Preparations of Platelet-Rich Plasma for the Treatment of Ocular Surface Disorders (E-PRP)
Alejandra E. Rodriguez, Sandra Gisbert, Antonio Palazón, Jorge L. Alio
Translational Vision Science & Technology. 2020; 9(6): 22
[Pubmed] | [DOI]
176 Novel Approaches in Addressing Ovarian Insufficiency in 2019: Are We There Yet?
Konstantinos Sfakianoudis, Anna Rapani, Sokratis Grigoriadis, Dimitra Retsina, Evangelos Maziotis, Petroula Tsioulou, Polina Giannelou, Konstantinos Pantos, Michael Koutsilieris, Nikolaos Vlahos, George Mastorakos, Mara Simopoulou
Cell Transplantation. 2020; 29: 0963689720
[Pubmed] | [DOI]
177 Comparison of efficiency between platelet rich plasma and corticosteroid injection therapies in patients with Carpal tunnel syndrome: a prospective randomized controlled study
Noha Ali Hashim, Hala Ahmed Fathy, Marwa Mohammed Esawy, Marwa Ahmed Shabana
The Egyptian Journal of Neurology, Psychiatry and Neurosurgery. 2020; 56(1)
[Pubmed] | [DOI]
178 Effect of autologous platelet-rich plasma transfusion in the treatment of infertile women with thin endometrium and its implications in IVF cycles: a literature review
Ahmed Samy, Ahmed M. Abbas, Ahmed Elmoursi, Mohamed Elsayed, Reda S. Hussein
Middle East Fertility Society Journal. 2020; 25(1)
[Pubmed] | [DOI]
179 Higher Physiologic Platelet Counts in Whole Blood Are Not Associated With Improved ACL Cross-sectional Area or Signal Intensity 6 Months After Bridge-Enhanced ACL Repair
Christina Freiberger, Ata M. Kiapour, Shanshan Liu, Rachael N. Henderson, Samuel Barnett, Nicholas J. Sant, Benedikt L. Proffen, Braden C. Fleming, Kirsten Ecklund, Dennis E. Kramer, Lyle J. Micheli, Martha M. Murray, Yi-Meng Yen
Orthopaedic Journal of Sports Medicine. 2020; 8(7): 2325967120
[Pubmed] | [DOI]
180 Platelet storage and functional integrity
Gianmatteo Vit, Harald Klüter, Patrick Wuchter
Journal of Laboratory Medicine. 2020; 44(5): 285
[Pubmed] | [DOI]
181 Role of Mechanical Loading for Platelet-Rich Plasma-Treated Achilles Tendinopathy
Alyssa Neph, Allison Schroeder, Keelen R. Enseki, Peter A. Everts, James H-C. Wang, Kentaro Onishi
Current Sports Medicine Reports. 2020; 19(6): 209
[Pubmed] | [DOI]
182 Regenerative Injectable Therapies: Current Evidence
William H. West, Anthony I. Beutler, Christopher R. Gordon
Current Sports Medicine Reports. 2020; 19(9): 353
[Pubmed] | [DOI]
183 Optimal activation methods for maximizing the concentrations of platelet-derived growth factor-BB and transforming growth factor-ß1 in equine platelet-rich plasma
Kentaro FUKUDA, Taisuke KURODA, Norihisa TAMURA, Hiroshi MITA, Yoshinori KASASHIMA
Journal of Veterinary Medical Science. 2020; 82(10): 1472
[Pubmed] | [DOI]
184 Optimal double-spin method for maximizing the concentration of platelets in equine platelet-rich plasma
Kentaro FUKUDA, Atsutoshi KUWANO, Taisuke KURODA, Norihisa TAMURA, Hiroshi MITA, Yuji OKADA, Yoshinori KASASHIMA
Journal of Equine Science. 2020; 31(4): 105
[Pubmed] | [DOI]
185 Effects of intraovarian injection of autologous platelet rich plasma on ovarian reserve and IVF outcome parameters in women with primary ovarian insufficiency
Yigit Cakiroglu, Ayse Saltik, Aysen Yuceturk, Ozge Karaosmanoglu, Sule Yildirim Kopuk, Richard T. Scott, Bulent Tiras, Emre Seli
Aging. 2020; 12(11): 10211
[Pubmed] | [DOI]

The Usefulness of Platelet-Rich Plasma (PRP) for the Treatment of Vitiligo: State of the Art and Review

Santo Raffaele Mercuri, Laura Vollono, Giovanni Paolino
Drug Design, Development and Therapy. 2020; Volume 14: 1749
[Pubmed] | [DOI]

Human Platelets and Derived Products in Treating Ocular Surface Diseases – A Systematic Review

Jingjing You, Chris Hodge, Monira Hoque, Constantinos Petsoglou, Gerard Sutton
Clinical Ophthalmology. 2020; Volume 14: 3195
[Pubmed] | [DOI]
188 Utilization of lyophilized platelet-rich plasma in esthetic facial treatment
Teh-Yang Cheng
Journal of Cosmetic Medicine. 2020; 4(2): 101
[Pubmed] | [DOI]
M. Krasnoselsky, O. Pushkar, L. Simonova, M. Myroshnychenko
???????? ??????????? ???????? ?? ????????????? = Problems of Radiation Medicine and Radiobiology. 2020; 25: 338
[Pubmed] | [DOI]
190 Live Birth in Woman With Premature Ovarian Insufficiency Receiving Ovarian Administration of Platelet-Rich Plasma (PRP) in Combination With Gonadotropin: A Case Report
Chao-Chin Hsu, Leonard Hsu, Isabel Hsu, Yi-Jen Chiu, Sonam Dorjee
Frontiers in Endocrinology. 2020; 11
[Pubmed] | [DOI]
191 The Stryker Regenkit PRP Kit Does Not Concentrate Canine Platelets
Barry Cherno, Leilani Alvarez, Kenneth E. Lamb, Sherman Canapp
Frontiers in Veterinary Science. 2020; 7
[Pubmed] | [DOI]
192 Short-Term Effects of Arthroscopic Microfracturation of Knee Chondral Defects in Osteoarthritis
Przemyslaw Krakowski, Robert Karpinski, Ryszard Maciejewski, Józef Jonak, Andrzej Jurkiewicz
Applied Sciences. 2020; 10(23): 8312
[Pubmed] | [DOI]
193 The Platelet Concentrates Therapy: From the Biased Past to the Anticipated Future
Tomoyuki Kawase, Suliman Mubarak, Carlos Fernando Mourão
Bioengineering. 2020; 7(3): 82
[Pubmed] | [DOI]
194 Anticoagulants Interfere With the Angiogenic and Regenerative Responses Mediated by Platelets
Paula Oneto, Paula Romina Zubiry, Mirta Schattner, Julia Etulain
Frontiers in Bioengineering and Biotechnology. 2020; 8
[Pubmed] | [DOI]
195 Importance of Timing of Platelet Lysate-Supplementation in Expanding or Redifferentiating Human Chondrocytes for Chondrogenesis
Margot Rikkers, Riccardo Levato, Jos Malda, Luciënne A. Vonk
Frontiers in Bioengineering and Biotechnology. 2020; 8
[Pubmed] | [DOI]
196 Impact of the Different Preparation Methods to Obtain Autologous Non-Activated Platelet-Rich Plasma (A-PRP) and Activated Platelet-Rich Plasma (AA-PRP) in Plastic Surgery: Wound Healing and Hair Regrowth Evaluation
Pietro Gentile, Claudio Calabrese, Barbara De Angelis, Laura Dionisi, Jacopo Pizzicannella, Ashutosh Kothari, Domenico De Fazio, Simone Garcovich
International Journal of Molecular Sciences. 2020; 21(2): 431
[Pubmed] | [DOI]
197 Systematic Review of Platelet-Rich Plasma Use in Androgenetic Alopecia Compared with Minoxidil®, Finasteride®, and Adult Stem Cell-Based Therapy
Pietro Gentile, Simone Garcovich
International Journal of Molecular Sciences. 2020; 21(8): 2702
[Pubmed] | [DOI]
198 Platelets in Healthy and Disease States: From Biomarkers Discovery to Drug Targets Identification by Proteomics
Erica Gianazza, Maura Brioschi, Roberta Baetta, Alice Mallia, Cristina Banfi, Elena Tremoli
International Journal of Molecular Sciences. 2020; 21(12): 4541
[Pubmed] | [DOI]
199 Systematic Review—The Potential Implications of Different Platelet-Rich Plasma (PRP) Concentrations in Regenerative Medicine for Tissue Repair
Pietro Gentile, Simone Garcovich
International Journal of Molecular Sciences. 2020; 21(16): 5702
[Pubmed] | [DOI]
200 Platelet-Rich Plasma Ameliorates Cyclophosphamide-Induced Acute Interstitial Cystitis/Painful Bladder Syndrome in a Rat Model
Yung-Hsiang Chen, Kee-Ming Man, Wen-Chi Chen, Po-Len Liu, Kao-Sung Tsai, Ming-Yen Tsai, Yu-Tzu Wu, Huey-Yi Chen
Diagnostics. 2020; 10(6): 381
[Pubmed] | [DOI]
201 Fluoride Modification of Titanium Surfaces Enhance Complement Activation
Maria H. Pham, Håvard J. Haugen, Janne E. Reseland
Materials. 2020; 13(3): 684
[Pubmed] | [DOI]
202 Human Fresh Fibrin Membrane with Bone Morphogenetic Protein-2 (BMP-2) Induces Bone Formation in the Subcutaneous Tissues of Nude Mice
Keiko Onji, Md. Arafat Kabir, Bowen Zhu, Kenji Yokozeki, Takashi Saito, Toshiyuki Akazawa, Masaru Murata
Materials. 2020; 14(1): 150
[Pubmed] | [DOI]
203 Important Considerations for Sample Collection in Metabolomics Studies with a Special Focus on Applications to Liver Functions
Lorraine Smith, Joran Villaret-Cazadamont, Sandrine P. Claus, Cécile Canlet, Hervé Guillou, Nicolas J. Cabaton, Sandrine Ellero-Simatos
Metabolites. 2020; 10(3): 104
[Pubmed] | [DOI]
204 Nanomechanical Properties of Articular Cartilage Due to the PRP Injection in Experimental Osteoarthritis in Rabbits
Mikhail Ihnatouski, Jolanta Pauk, Boris Karev, Dmitrij Karev
Molecules. 2020; 25(16): 3734
[Pubmed] | [DOI]
205 Proteomic Classification and Identification of Proteins Related to Tissue Healing of Platelet-Rich Plasma
Ho Won Lee, Kyung-Ho Choi, Jung-Youn Kim, Kyung-Ok Kim, Bai Haotian, Liu Yuxuan, Kyu-Cheol Noh
Clinics in Orthopedic Surgery. 2020; 12(1): 120
[Pubmed] | [DOI]
206 Extraction socket preservation using a collagen plug combined withplatelet-rich plasma (PRP): A comparative clinico-radiographic study
Numaan Nisar, Kumar Nilesh, Mushtaq Ishaq Parkar, Prashant Punde
Journal of Dental Research, Dental Clinics, Dental Prospects. 2020; 14(2): 139
[Pubmed] | [DOI]
207 Recovery therapy after photodynamic therapy of background and precancerous diseases of the vulva
V.V. Dunaevskaya, T.F. Tatarchuk, O.V. Zanko
Medicine Today and Tomorrow. 2020; 86(1): 69
[Pubmed] | [DOI]
208 A comparative study on the efficacy of fractional CO2 laser and fractional CO2 laser with autologous platelet-rich plasma in scars
Satish Godara, Sandeep Arora, Rajeshwari Dabas, Gulhima Arora, Gopi Renganathan, Richa Choudhary
Indian Dermatology Online Journal. 2020; 11(6): 930
[Pubmed] | [DOI]
209 Comparison of platelet-rich plasma prepared using two methods: Manual double spin method versus a commercially available automated device
Vishal Gupta, AnitaS Parihar, Mona Pathak, VinodK Sharma
Indian Dermatology Online Journal. 2020; 11(4): 575
[Pubmed] | [DOI]
210 Platelet-rich Blood Derivatives for Tendon Regeneration
Raquel Costa-Almeida, Pedro S. Babo, Rui L. Reis, Manuela E. Gomes
Journal of the American Academy of Orthopaedic Surgeons. 2020; 28(5): e202
[Pubmed] | [DOI]
211 Platelet-Rich Plasma and Epidural Platelet Lysate: Novel Treatment for Lumbar Disk Herniation
Benjamin Rawson
Journal of Osteopathic Medicine. 2020; 120(3): 201
[Pubmed] | [DOI]
212 The optimal platelet concentration in platelet-rich plasma for proliferation of human cells in vitro—diversity, biases, and possible basic experimental principles for further research in the field: A review
Olav K. Straum
PeerJ. 2020; 8: e10303
[Pubmed] | [DOI]
213 Turn down - turn up: a simple and low-cost protocol for preparing platelet-rich plasma
Edilson Silva Machado, Renata Leite, Cintia Cichowski dos Santos, Georgia Lazzari Artuso, Fernando Gluszczak, Leonardo Giovani de Jesus, José Manuel Peixoto Caldas, Markus Bredemeier
Clinics. 2019; 74: e1132
[Pubmed] | [DOI]
214 Osteo/odontogenic Differentiation of Human Mesenchymal Stem Cells with Platelet-rich Plasma and Mineral Trioxide Aggregate
Shanthi Vanka, Amit Vanka, Sandeep Kumar Vishwakarma, Manohar K Bhat, Othman Wali, Aleem A Khan
The Journal of Contemporary Dental Practice. 2019; 20(10): 1171
[Pubmed] | [DOI]
Sergiy Chetverikov, Dmitro Atanasov
EUREKA: Health Sciences. 2019; 1: 3
[Pubmed] | [DOI]
Sergiy Chetverikov, Dmitro Atanasov
EUREKA: Health Sciences. 2019; 2: 11
[Pubmed] | [DOI]
217 Platelet-rich plasma improves impaired glucose hemostasis, disrupted insulin secretion, and pancreatic oxidative stress in streptozotocin-induced diabetic rat
Marzieh Zarin, Narges Karbalaei, Sara Keshtgar, Marzieh Nemati
Growth Factors. 2019; 37(5-6): 226
[Pubmed] | [DOI]
218 Stability of the Human Plasma Proteome to Pre-analytical Variability as Assessed by an Aptamer-Based Approach
Jaclyn R. Daniels, Zhijun Cao, Mackean Maisha, Laura K. Schnackenberg, Jinchun Sun, Lisa Pence, Thomas C. Schmitt, Beate Kamlage, Sarah Rogstad, Richard D. Beger, Li-Rong Yu
Journal of Proteome Research. 2019; 18(10): 3661
[Pubmed] | [DOI]
219 The use of platelet-rich plasma to treat chronic tendinopathies: A technical analysis
Jean-François Kaux, Thibault Emonds-Alt
Platelets. 2018; 29(3): 213
[Pubmed] | [DOI]
220 A Review of Platelet-Rich Plasma: History, Biology, Mechanism of Action, and Classification
Rubina Alves, Ramon Grimalt
Skin Appendage Disorders. 2018; 4(1): 18
[Pubmed] | [DOI]
221 Effectiveness of Platelet-Rich Plasma for Androgenetic Alopecia: A Review of the Literature
Jessica Cervantes, Marina Perper, Lulu L. Wong, Ariel E. Eber, Alexandra C. Villasante Fricke, Tongyu Cao Wikramanayake, Joaquin J. Jimenez
Skin Appendage Disorders. 2018; 4(1): 1
[Pubmed] | [DOI]
222 Platelet-Rich Plasma in Orthopaedic Surgery
Dean Wang, Scott A. Rodeo
JBJS Reviews. 2017; 5(9): e7
[Pubmed] | [DOI]
223 Tissue engineering in orthopaedic sports medicine: current concepts
João Bebiano Costa, Helder Pereira, João Espregueira-Mendes, Gilson Khang, Joaquim Miguel Oliveira, Rui Luis Reis
Journal of ISAKOS. 2017; 2(2): 60
[Pubmed] | [DOI]
224 Concise Review: The Use of Adipose-Derived Stromal Vascular Fraction Cells and Platelet Rich Plasma in Regenerative Plastic Surgery
Pietro Gentile, Maria Giovanna Scioli, Alessandra Bielli, Augusto Orlandi, Valerio Cervelli
STEM CELLS. 2017; 35(1): 117
[Pubmed] | [DOI]
225 Applications of platelet-rich plasma in dermatology: A critical appraisal of the literature
M. D. Lynch, S. Bashir
Journal of Dermatological Treatment. 2016; 27(3): 285
[Pubmed] | [DOI]


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